A Citrullus colocynthis fruit extract acutely enhances insulin-induced GLUT4 translocation and glucose uptake in adipocytes by increasing PKB phosphorylation

Citrullus colocynthis (L.) Schrad is a common fruit in traditional medicine and used as remedy against various diseases, especially diabetes. Up to now, its anti-diabetic effects have been fully attributed enhancement of pancreatic insulin secretion. Whether C. also ameliorates action peripheral tissues has not investigated. In the present study, using 3T3-L1 adipocytes cell model, we investigated whether colocynth extracts affect action. Various were prepared from screened cell-based 96 well plate GLUT4 translocation assay. Promising further studied for their on glucose uptake viability. The effect signal transduction was determined by Western blot molecular composition established LC-MS. ethyl acetate fractions aqueous non-defatted seed pulp, designated Sna1 Pna1, acutely enhanced insulin-induced translocation. accordance, both increased insulin-stimulated cellular uptake. which displayed greater than Sna1, demonstrated increase without changing half-maximum dose (ED50) insulin, nor kinetics. At level, Pna1 found enhance PKB phosphorylation receptor. appeared be toxic cells and, like restored viability during serum starvation. By investigating nine compounds identified that made up 87% mass extract, one likely responsible insulin-enhancing Pna1. possesses activity. This activity may explain part medicine. It identifies source potential novel enhancer prove useful reduce hyperglycemia type 2